Choosing the right cell freezing method
The current recommendation for freezing stem cells, primary cells, PBMCs and cell lines is that they be cooled gradually to approximately -80°C at a controlled rate of -1°C per minute. This can be achieved using CoolCell® alcohol-free cell freezing container in conjunction with a -80°C freezer.
Learn how with this short video.
Fig 1. CoolCell performance compared to a controlled-rate freezer
Multiple leukapheresis-like products were processed into PBMCs and frozen using either a CoolCell or controlled-rate freezer (CRF). In both cases, cells were frozen at approximately 108 cells per mL in CryoStor® CS10. Vials were stored in liquid nitrogen for approximately 2-3 weeks and thawed the same day using a water bath. The CoolCell and CRF methods were comparable in both recovery and viability. (Data generated by UCSF Division of Transplant Surgery)
TxCell, SA (Valbonne, France). In a feasibility study for their Ovasave® clinical trials, France-based TxCell, SA determined CoolCell cell freezing containers performed equivalent to a controlled-rate freezer (CRF) for the development of of their cell therapy products. (Figure 2). Because CoolCell is considerably easier to deploy to clinical sites, is more cost-effective and requires no maintenance, it was adopted over a CRF for the Ovasave phase 2b trial. (Please visit our Cell Therapy Processes page for more information on the TxCell study.) Learn how TxCell qualified and adopted CoolCell for their Treg clinical trial.
Fig 2. CoolCell performance compared to a controlled-rate freezer
Performance test: Effects of freezing on antigen-specific Treg (Ag-Treg) cell therapy products; Ag-Tregs (n = 6) were frozen at a concentration of 1 to 10 × 106 cells/mL using the CoolCell freezing device or programmable freezer (freezing rate of –1 °C/min). Viability and absolute viable cell count of thawed Ag-Treg cell therapy products were evaluated by flow cytometry. (Data generated by TxCell, SA)
CoolCell outperforms other passive freezing methods
An independent study conducted by Roslin Cellab, showed a 33 percent increase in hESC viable cell count using a CoolCell container (P<0.005) when compared to other passive freezing methods three days post-thaw (Figure 3).
Fig 3. CoolCell performance compared to a controlled-rate freezer
Post-thaw viability of Human Embryonic Stem Cells (RC-10): Human embryonic stem cells, RC-10, grown to confluence were harvested and suspended in a cryopreservative at 2 x 106 cells/vials and then frozen in 1.0 ml cryogenic vials using the methods above. After at least two weeks in LN2, 3 vials from each treatment were thawed and cells were counted immediately (Day 1) or after three days growth (Day 3). The CoolCell freezing method led to a slight increase in the number of viable cells on day 1 and a 33% increase in the number of cells after 3 days of growth. (Data provided by Roslin Cellab, Scotland)
How do CoolCell cell freezing containers compare to other freezing methods?
• Consistent -1˚C/minute freeze rate to all vials
• Stated freeze rate of -1°C/minute, but varies based on vial position
How do CoolCell containers work?
CoolCell freezing containers are passive devices that provide a controlled -1℃/minute freeze rate to all cryogenic vials when placed in a -80℃ freezer. CoolCell containers do not require isopropanol or any fluids to regulate heat removal and provide uniform and reproducible freezing to all vials. (Figure 4) See more information here.
Choose the right CoolCell cell freezing container for your application
- CoolCell LX for 12 x 1.8 or 2.0 mL cryogenic vials
CoolCell FTS30 for 30 x 1.8 or 2.0 mL cryogenic vials
- CoolCell 5ml LX for 12 x 5.0 mL cryogenic vials
- CoolCell SV2 for 12 x 2.0 mL injectable ampules
- CoolCell SV10 for 6 x 10.0 mL injectable ampules
More Customer Reviews
"We run a registry in which large amounts of PBMCs are processed for long-term cryopreservation. After testing
the CoolCell, we found slightly better cell viability (>90%) than our current cell freezing containers, and there is
no isopropanol waste generated. Overall, the CoolCell has proven to set a new bar in cryopreservation."
- Rohit Gupta, Stanford University
"We have been using a cell freezing system which requires 100% isopropyl for the stepwise (1°C/min) cooling of cell lines for cryopreservation. This required the regular and frequent changes of the isopropyl alcohol to maintain the unit's performance, due to evaporation and changes in concentration of the isopropyl alcohol. As soon as we used
the BioCision CoolCell, its ease of use and the low (zero) maintainence of the unit were quickly apparent to us.
This is by far and away the best bench top technology to enter the field of cell cryopreservation for decades."
- John Gardner, Roslin Cellab Project Leader, Scotland