Protocols

Controlled-Rate Cell Cryopreservation

CoolCell® delivers consistent freezing profiles to all cryogenic tubes and each freeze batch.  Consistent results ensure successful cryopreservation and reproducibly high post-thaw cell viability and function. CoolRack®, CoolBox™, and ThermalTray™ also aid in maintaining sterility during the sample preparation and thawing processes.

Controlled-Rate Cell Cryopreservation Using a Dry Ice Locker

Product ImageWhen a -80°C freezer isn't available, the CoolCell® cell freezing systems can be successfully used in a dry ice locker to achieve reproducible and viable cell freezing. See testimonial below:

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"Purely altruistically, I wanted to mention that one big advantage of the CoolCell is that you can use dry ice to do your freezing if you don't have a -80. I spent nearly a year getting terrible viability-recovery on cell freezing with dry ice (in an alcohol device) followed by storage in LN2, and this on HeLa cells which aren't exactly hard to store. I then bought a CoolCell and now I can just use standard cryo-preservation techniques (DMSO, 20% NBS) and get nearly 100% viability on thawing. Sam Knight - Ceramisphere (Australia)

 


General Snap-Freezing

Snap-freezing with a CoolRack® or CoolSink™ thermo-conductive module provides an efficient, alcohol-free and highly reproducible method.  The properties of the alloy material ensure each sample tube or well experiences the same temperature profile - whether on dry ice or in liquid nitrogen.  See performance data for information on snap-freezing rates and standard deviation reduction when using a CoolRack compared to directly inserting tubes into dry ice, dry ice / alcohol baths or liquid nitrogen.

Virus Cryopreservation

Freezing viral samples with a pre-cooled CoolRack® ensures rapid, consistent and reproducible freezing.  The CoolRack method is a safe and efficient replacement to the dry-ice/alcohol slurry freezing method - eliminating the risks of contamination and variation in molecular integrity, while still producing high virus titer levels .


Bacteria Cryopreservation

Freezing bacterial samples with a pre-cooled CoolRack® ensures rapid, consistent and reproducible freezing.  The CoolRack method is a safe and efficient replacement to the dry-ice/alcohol slurry freezing method - eliminating the risks of contamination and variation in molecular integrity, while still producing high bacteria titer levels.

 

 

Application Notes

Ice-Free Cooling and Freezing

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CoolRack® tube modules and CoolSink™ plate modules can be used in the ice-free CoolBox Systems for hours of benchtop ice-free cooling and freezing.  Reusable cartridges ensure sample temperature uniformity - no edge effect - in a compact workspace. Highly durable and versatile.

Incubating and Thawing in a Water Bath

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CoolRack® tube modules and CoolSink™ plate modules may be placed on a ThermalTray™ platform for use in a water bath.  Ensure tubes and plates stay at desired temperature, but eliminate risks associated with direct water contact.

Multichannel Pipetting Assembly with Constant Temperature Control

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CoolRack M96ID 96-well 1.5ml/2.0ml thermo-conductive tube module features wells spaced to fit an adjustable-head 8-channel pipetter.  Transferring from tubes to a multi-well plate has never been easier or more reproducible.