Protocols

Featured Article

See also Reviews and Publications

Featured Article

Standardized Cryopreservation of Pluripotent Stem Cells
Rick I. Cohen, Maria L. Thompson,Brian Schryver, Rolf O. Ehrhardt
Current Protocols in Stem Cell Biology, November 2015

 

The successful exploitation of human cells for research, translational, therapeutic, and commercial purposes requires that effective and simple cryopreservation methods be applied for storage in local and master cell banks. Of all cell types utilized in modern research, human embryonic stem cells and their more recent relatives, induced pluripotent stem cells, are two of the most sensitive to cryopreservation. It is frequently observed that the lack of quality control and proper processing techniques yield poor recovery of pluripotent stem cells. The procedures in this unit have been optimized for handling some of the most recalcitrant stem cell lines, and provide a method for controlled-rate freezing, using minimal equipment that affords levels of cell viability comparable to expensive controlled-rate freezers, and standardized cell thawing. The protocol provides a clinically relevant, inexpensive, reliable, and user-friendly method that successfully prepares cells for long-term cold storage and ensures maximum levels of cell viability post thaw.

Protocols

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of published protocols from the growing community of BioCision customers, external collaborators, and internal scientists.

Cell and Stem Cryopreservation

Standardized Cryopreservation of Human Primary Cells
Thomas V. Ramos, Aby J. Mathew, Maria L. Thompson, Rolf O. Ehrhardt
Current Protocols in Cell Biology, November 2014
Product Featured: CoolCell

Standardized Cryopreservation of Pluripotent Stem Cells
Rick I. Cohen, Maria L. Thompson,Brian Schryver, Rolf O. Ehrhardt
Current Protocols in Stem Cell Biology, November 2015
Product Featured: CoolCell

Cryopreservation and Thawing of Cells
Wayne M. Yokoyama, Maria L. Thompson, Rolf O. Ehrhardt
Current Protocols in Immunology, November 2012
Product Featured: CoolCell

Using CoolCell Cell Freezing Container in a Dry Ice Locker to Cryopreserve Cells
Maria Thompson, Brian Schryver, Rolf Ehrhardt
BioCision LLC, 2011
Product Featured: CoolCell

Rate-controlled Cryopreservation and Thawing of Mammalian Cells
Maria Thompson, Michelle Nemits, and Rolf Ehrhardt
Nature: Protocol Exchange, March 2011
Product Featured: CoolCell

Reproducible Cell Freezing
The Biomedical Scientist
British Journal of Biomedical Science, August 2010
Product Featured: CoolCell

New CoolCell Cell Freezing Container Video from BioCision
LabGrab, February 2010
Product Featured: CoolCell

CoolCell - Controlled Cell Freezing w/o Alcohol or Maintenance Cost
Rolf Ehrhardt, Brian Schryver and Jeff Schryver
Journal of Visualized Experiments (JoVE),
2010
Product Featured: CoolCell

PBMC Isolation

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Isolation of Human Mononulcear Cells from Blood
Mark Klein, Professor Dr. A.B.J Prakken, Ucan-U, November 2011
Product Featured: CoolCell

Cross Network PBMC Processing SOP
Cross-Network PBMC SOP Working Group, April 2009
Product Featured: CoolCell

Virus Cryopreservation

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Virus Cryopreservation Protocol
Aurelio Bonavia, Maria Thompson, and Rolf Ehrhardt, Protocol Exchange
February 2012
Product Featured: CoolRack

Virus Cryopreservation using a CoolRack Module
Aurelio Bonavia, Maria Thompson, and Rolf Ehrhardt, BioCision LLC, 2012
Product Featured: CoolRack CFT30, CoolBox CFT30 System, ThermalTray LP, CoolSink LX55, TruCool Cryogenic vials, TruCool Hinged CryoBoxes

 

Bacteria Cryopreservation

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Bacteria Cryopreservation Protocol
Aurelio Bonavia, Maria Thompson, Brian Schryver, and Rolf Ehrhardt
Protocol Exchange
, March 2012
Product Featured: CoolRack

Bacteria Cryopreservation using a CoolRack Module
Aurelio Bonavia, Maria Thompson, Brian Schryver, and Rolf Ehrhardt
BioCision LLC, 2011
Product Featured:CoolBox CFT30 System, CoolBox 30 Freezing Cartridge, CoolRack CFT30, TruCool Cryogenic vials, CryoLabels, CoolSink, ThermalTray LP, CoolSink LX55

 

Tissue Snap freezing

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Snap Freezing of Tissue Samples
Maria Thompson, Brian Schryver, Rolf Ehrhardt, BioCision LLC, 2011
Product Featured: CoolRack M15-PF, CoolRack M-30 PF, CoolBox 30 System, TruCool microcentrifuge tubes, CoolMat, ThermalTray LP

Snap-Freezing in Liquid Nitrogen Using a CoolRack module and
ThermalTray platform

Maria Thompson, Brian Schryver, Rolf Ehrhardt, BioCision LLC, 2011
Product Featured: CoolRack, ThermalTray

Snap Freezing Samples in Dry Ice using a CoolRack Module
Maria Thompson, Brian Schryver, Rolf Ehrhardt, BioCision LLC, 2011
Product Featured: CoolRack, CoolBox 30

 

Other

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Cell Culture Techniques for In Vivo Grafting
In Vivo Imaging Community, 2012
Product Featured: CoolCell

Protocol for Cryopreservation of Nucleofection™ Competent Cells
Lonza Cologne GmbH, BioResearch, 2012
Product Featured: CoolCell

Using a CoolRack M96ID Module with a Multi-Channel Pipetter
Brian Schryver and Rolf Ehrhardt, BioCision LLC, May 2012
Product Featured: CoolRack M96ID, 9L Ice Pan

Waterbaths with ThermalTray, CoolRack and CoolSink
Brian Schryver and Dr. Ehrhardt, BioCision LLC, May 2012
Product Featured: ThermalTray LP, ThermalTray HP, CoolRack, CoolSink

The Ice-Bucket Makeover - Portable Laboratory Cooling Gets with the Times
Maria Thompson and Dr. Ehrhardt, BioCision LLC, May 2010
Product Featured:CoolRack PCR, CoolRack M96ID, CoolBox CFT30 System