Publications and Protocols

Featured Article

Featured Article

Standardized Cryopreservation of Pluripotent Stem Cells
Cohen RI, Thompson ML, Schryver B, Ehrhardt RO
Current Protocols in Stem Cell Biology, March 2014

The successful exploitation of human cells for research, translational, therapeutic, and commercial purposes requires that effective and simple cryopreservation methods be applied for storage in local and master cell banks. Of all the cell types utilized in modern research, human embryonic stem cells and their more recent relatives, induced pluripotent stem cells, are two of the most sensitive to cryopreservation. It is frequently observed that the lack of quality control and proper processing techniques yield poor recovery of pluripotent stem cells. The procedures in this unit have been optimized for handling some of the most recalcitrant stem cell lines, and provide a method for controlled-rate freezing, using minimal equipment that affords levels of cell viability comparable to expensive controlled-rate freezers.

Protocols

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of published protocols from the growing community of BioCision customers, external collaborators, and internal scientists.

Cell and Stem Cryopreservation

Standardized Cryopreservation of Pluripotent Stem Cells
Cohen RI, Thompson ML, Schryver B and Ehrhardt RO
Current Protocols in Stem Cell Biology, March 2014
Product Featured: CoolCell

Cryopreservation and Thawing of Cells
Yokoyama WM, Thompson ML, and Ehrhardt RO,
Current Protocols in Immunology
, November 2012
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Rate-Controlled Cryopreservation and Thawing of Mammalian Cells
Maria Thompson, Brian Schryver, Rolf Ehrhardt
BioCision LLC, 2011
Product Featured: CoolCell

Using CoolCell Cell Freezing Container in a Dry Ice Locker to Cryopreserve Cells
Maria Thompson, Brian Schryver, Rolf Ehrhardt
BioCision LLC, 2011
Product Featured: CoolCell

Rate-controlled Cryopreservation and Thawing of Mammalian Cells
Maria Thompson, Michelle Nemits, and Rolf Ehrhardt
Nature: Protocol Exchange, March 2011
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Reproducible Cell Freezing
The Biomedical Scientist
British Journal of Biomedical Science, August 2010
Product Featured: CoolCell

Cell Snapshot - Reproducibility in cryopreservation is a necessary step towards consistent post-thaw cell function
Rolf Ehrhardt, MD, PhD and Brian Schryver
BioCision LLC, February 2010
Product Featured: CoolCell

New CoolCell Cell Freezing Container Video from BioCision
LabGrab, February 2010
Product Featured: CoolCell

CoolCell - Controlled Cell Freezing w/o Alcohol or Maintenance Cost
Rolf Ehrhardt, Brian Schryver and Jeff Schryver
Journal of Visualized Experiments (JoVE),
2010
Product Featured: CoolCell

PBMC Isolation

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Isolation of Human Mononulcear Cells from Blood
Mark Klein, Professor Dr. A.B.J Prakken, Ucan-U, November 2011
Product Featured: CoolCell

Cross Network PBMC Processing SOP
Cross-Network PBMC SOP Working Group, April 2009
Product Featured: CoolCell

Virus Cryopreservation

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Virus Cryopreservation Protocol
Aurelio Bonavia, Maria Thompson, and Rolf Ehrhardt, Protocol Exchange
February 2012
Product Featured: CoolRack

Virus Cryopreservation using a CoolRack Module
Aurelio Bonavia, Maria Thompson, and Rolf Ehrhardt, BioCision LLC, 2012
Product Featured: CoolRack CFT30, CoolBox CFT30 System, ThermalTray LP, CoolSink LX55, TruCool Cryogenic vials, TruCool Hinged CryoBoxes

 

Bacteria Cryopreservation

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Bacteria Cryopreservation Protocol
Aurelio Bonavia, Maria Thompson, Brian Schryver, and Rolf Ehrhardt
Protocol Exchange
, March 2012
Product Featured: CoolRack

Bacteria Cryopreservation using a CoolRack Module
Aurelio Bonavia, Maria Thompson, Brian Schryver, and Rolf Ehrhardt
BioCision LLC, 2011
Product Featured:CoolBox CFT30 System, CoolBox 30 Freezing Cartridge, CoolRack CFT30, TruCool Cryogenic vials, CryoLabels, CoolSink, ThermalTray LP, CoolSink LX55

 

Tissue Snap freezing

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Snap Freezing of Tissue Samples
Maria Thompson, Brian Schryver, Rolf Ehrhardt, BioCision LLC, 2011
Product Featured: CoolRack M15-PF, CoolRack M-30 PF, CoolBox 30 System, TruCool microcentrifuge tubes, CoolMat, ThermalTray LP

Snap-Freezing in Liquid Nitrogen Using a CoolRack module and
ThermalTray platform

Maria Thompson, Brian Schryver, Rolf Ehrhardt, BioCision LLC, 2011
Product Featured: CoolRack, ThermalTray

Snap Freezing Samples in Dry Ice using a CoolRack Module
Maria Thompson, Brian Schryver, Rolf Ehrhardt, BioCision LLC, 2011
Product Featured: CoolRack, CoolBox 30

 

Other

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Cell Culture Techniques for In Vivo Grafting
In Vivo Imaging Community, 2012
Product Featured: CoolCell

Protocol for Cryopreservation of Nucleofection™ Competent Cells
Lonza Cologne GmbH, BioResearch, 2012
Product Featured: CoolCell

Using a CoolRack M96ID Module with a Multi-Channel Pipetter
Brian Schryver and Rolf Ehrhardt, BioCision LLC, May 2012
Product Featured: CoolRack M96ID, 9L Ice Pan

Waterbaths with ThermalTray, CoolRack and CoolSink
Brian Schryver and Dr. Ehrhardt, BioCision LLC, May 2012
Product Featured: ThermalTray LP, ThermalTray HP, CoolRack, CoolSink

The Ice-Bucket Makeover - Portable Laboratory Cooling Gets with the Times
Maria Thompson and Dr. Ehrhardt, BioCision LLC, May 2010
Product Featured:CoolRack PCR, CoolRack M96ID, CoolBox CFT30 System

Publications

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of publications from the growing community of BioCision customers, external collaborators, and internal scientists.

Playing it Cool
Rolf Ehrhardt, MD, PhD and Maria Thompson, PhD
EBR, September 2014

Select Stem Cells and T Cells Publications Citing BioCision Products
BioCision, LLC. July 2014
Product Featured: CoolCell

Understanding the Effects of Cryopreservation on Cellular Therapy: A Study of Mesenchymal Stromal Cells in the Clinic
Guido Moll, Jessica Alm, Lindsay Davis, Lena von Bahr, Nina Heldring, Lillemor Stenbeck-Funke, Osama Hamad, Robin Hinsch, Lech Ignatowicz, Matthew Locke, Helena Lonnies, John Lambris, Yuji Teramura, Kristina Nilsson-Ekdahl, Bo Nilsson and Katerina Le Blanc
Stem Cells: Translational and Clinical Research, April 2014
Product Featured: CoolCell

Use of a Low-Cost Passive Freezing Device in the Effective Cryopreservation and Recovery of Human T-regs for use in a Cell Therapy Trial
Arnaud Foussat, Rodney Rietze, Maria Thompson, Brian Schryver, and Rolf Ehrhardt Poster #78 International Society of Cell Therapy, April 2014
Product Featured: CoolCell

Validation of a Novel Portable Freezing Device in the Optimal Freezing  of Peripheral Blood Mononuclear Cells for Potential Cell Therapy Use
Amy Putnam, Eleonora Trotta, Maria Thompson, Brian Schryver, and Rolf Ehrhardt Poster #79 International Society of Cell Therapy, April 2014
Product Featured: CoolCell

Temperature Standardization Gets the Cold Shoulder
Maria Thompson, Brian Schryver, Rolf Ehrhardt, Laboratory Equipment
December 2013
Product Featured: CoolCell

Directed differentiation of human pluripotent cells to neural crest stem cells
Laura Menendez, Michael Kulik, Austin Page, Sarah Park, James Lauderdale, Michael Cunningham, Stephen Dalton, Nature Protocols, January 2013
Product Featured: CoolCell

New Method for Cryopreservation of Fresh Water Diatoms
Buhmann MT, Day JG, and Kroth PG. “Post-cryopreservation viability of the benthic freshwater diatom Planothidium frequentissimum depends on light levels.” Cryobiology, 2013. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Cellular stress research is aided by CoolCell freezing container
Marunde, MR. "Improved tolerance to salt and water stress in Drosophila melanogaster cells conferred by late embryogenesis abundant protein." J Insect Physiol. 2013 Apr; 59(4):377-86. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Promise for parkinsons cell therapy
Hu Xu. et al. “Neurotrophic factor expression in expandable cell populations from brain samples in living patients with Parkinson’s disease.” The FASEB Journal: Research Communication. 2013. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Sample Handling: Quality Assurance Could Improve Biomarker Search
Geddes, TJ. et al. "SPIN: Development of Sample-specific Protein Integrity Numbers as an Index of Biospecimen Quality." Biopreservation and Biobanking. 2013 Vol 11(1):25-32. DOI: 10.1089/bio.2012.0039. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Fieldwork Sample Handling is Improved by CoolCell Freezing Container
Kwok A. et al. "Cryopreservation and storage of mussel (Mytilus spp.) haemocytes for latent analysis by the Comet assay." Mutation Research 750 (2013) 86-91
The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks
Yichen Shi, Peter Kirwan & Frederick J Livesey, Nature Protocols, September 2012
Product Featured: CoolCell

Standardization of the Cryopreservation Process for Parathyroid Glands
BioKryo GmbH, Fraunhofer IBMT, The 12th Cryogenics, 2012
Product Featured: CoolCell

Neuronal stem cells are cryopreserved with CoolCell freezing container
Shei, Y. et al. "Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks." Nature Protocols. 2012 Oct;7(10):1836-46. doi: 10.1038/nprot.2012.116.
The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Precision in Cryopreservation- Equiptment and Control
By Stephen Butler and David Pegg, University of York, March 2012
Product Featured: CoolCell

Neural Crest Stem Cell Cryopreservation
Shei, Y. et al. "Directed differentiation of human pluripotent stem cells to cerebral cortex neurons and neural networks." Nature Protocols. 2012 Oct;7(10):1836-46. doi: 10.1038/nprot.2012.116. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Using PARP Inhibitor in Cancer Therapeutics
Shall, S. et al. “The Use of PARP Inhibitors in Cancer Therapy: Use as Adjuvant with Chemotherapy or Radiotherapy; Use as a Single Agent in Susceptible Patients; Techniques Used to Identify Susceptible Patients” 2011. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Isolation on PBMC
Network for Pancreatic Organ Donors with Diabetes, May 2011
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Isolation of Cells from Spleen, Thymus and Lymph Nodes
Network for Pancreatic Organ Donors with Diabetes, May 2011
Product Featured: CoolCell

Isolation and preservation of peripheral blood mononuclear cells for analysis of islet antigen-reactive T cell responses
R Mallone, S.I. Mannering, BM Brooks - Worrell, I Durinovic-Bello, C Cillo, F.S. Wong, N.C. Schloot, Journal of Clinical and Experimental Immunology, 2011. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

CoolCell Endorsed for Freezing Antibody-Producing Clones
Dharshanan S. "Application of cytotechnology techniques: A case study for the production, purification and characterization of humanized antibody secreted by NS0 transfectoma." AsPac J. Mol. Biol. Biotechnol. 2011. Vol 19 (2): 63-71. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

The Ice-Bucket Makeover - Portable Laboratory Cooling Gets with the Times
Mr. Schryver and Dr. Ehrhardt, BioCision LLC, May 2010
Product Featured:CoolRack PCR, CoolRack M96ID, CoolBox CFT30 System

Drug Discovery and Cardiotoxicity: CHO and HEK cells
Xueying, C. et al. “Cardiac ion channel safety profiling on the ionworks quattro automated patch clamp system” ASSAY and Drug Development Technologies, 2010.
The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Other

Researchers all across the globe are successfully using our products for a wide range of applications. We are proud to share this list of published material from the growing community of BioCision customers, external collaborators, and internal scientists.

Freezing Containers for Cell Preservation
Maria Thompson, PhD. GEN: Genetic Engineering and Biotechnology News
June 2014
Product Featured: CoolCell

A Meeting of the Minds for Sample Management
DDN News, May 2014 Volume 10, Issue 5

Back to the Future
R. Lee Buckler, Rolf Ehrhardt, Maria Thompson
EBR, April 2014
Product Featured: CoolCell

BioBanking Technologies: A Global Market Watch 2009-2015
Axis Research Mind, November 2011
Product Featured: CoolCell

Application of cytotechnology techniques: A case study for the production, purification and characterization of humanized antibody secreted by NS0 transfectoma
Suba Dharshanan, Heilly Chong, Cheah Swee Hung and Zulkeflie Zamrod, June 2011. The paper above has been summarized on the biocision blog
Product Featured: CoolCell

Safeguarding Biological Specimen Integrity
Carol Potera, Genetic and Engineering News, February 2011
Product Featured: CoolBox, CoolCell, CoolRack

Cell Snapshot - Reproducibility in cryopreservation is a necessary step towards consistent post-thaw cell function
Rolf O. Ehrhardt, MD, PhD and Brian Schryver, Laboratory Equipment, February 2010
Product Featured: CoolCell

Reproducible Cell Assays
Brian Schryver and Rolf O. Ehrhardt, MD, PhD, G.I.T Laboratory Journal
September 2010
Product Featured: CoolCell

Improved thermal management of microtiter plates using the BioCision CoolSink
Brian Schryver and Rolf O. Ehrhardt, MD, PhD BioTechniques, November 2010
Product Featured: CoolSink 96F

Reducing the variability of pre-analytical handling of biological samples
BioCision Biotech International, November 2010
Product Featured: CoolRack PF 500, ThermalTray

Top Trends In Life Science Research
Bioscience Technology, June 2010
Product Featured: CoolCell

Reproducible Cell Freezing The Biomedical Scientist
British Journal of Biomedical Science
Pathology in Practice
Brian Schryver and Rolf O. Ehrhardt, MD, PhD, The Biomedical Scientist, August 2010
Product Featured: CoolCell

CellPlayer™ 96-well Kinetic Cell Migration and Invasion Assay
Meagan Roddy,Thom Nelson, Daniel M. Appledom and Vince Groppi, Essen BioScience Application Note, 2012
Product Featured: CoolSink

Mature Oocyte Cryopreservation: A Guideline
American Society for Reproductive Medicine, 2012

The Optimal Stage of Freezing Human Embryos
Leniaud Louis and Sifer Christophe, Current Women's Health Review, January 2009

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