Technology and Performance Data

BioCision products provide innovative alternatives to out-dated methods and tools, enabling you to minimize variability in your research and standardize common temperature-sensitive laboratory procedures.  BioCision products feature advanced thermal management technologies and functional design to address and support the workflow needs of pre-analytical sample handling, bioprocessing, biobanking, and cold-chain logistics.

 

CoolCell®
Cell cryopreservation products

 

CoolCell® alcohol-free cell cryopreservation products are the standard for reproducible passive controlled-rate cell freezing. When placed in a -80°C freezer, CoolCell containers will regulate the removal of sample heat at a rate of -1°C/minute, ensuring consistent and reproducible cryopreservation for a variety of cells.  The patent-pending CoolCell technology is used for a variety of cell types, including stem cells, primary cells, PBMC, cell lines, yeast, bacteria, insect cells and others. The CoolCell freezing method replaces out-dated methods that require isopropanol-based containers.  CoolCell cryopreservation containers perform as well or better than expensive programmable freezers, yet require no programming or maintenance which ensures a more standardized cryopreservation regimen.  

CoolCell containers may be used in -80°C freezers or dry ice chambers if mobile cryopreservation is required.

 

HOW IT WORKS



A CoolCell LX container, in combination with a -80°C freezer, will provide the freezing rate of -1°C/minute that is ideal for cryopreservation of most cells and cell lines. The container uses a combination of uniform-density cross-linked polyethylene insulative foam, a metal alloy core ring, and radial vial symmetry. As a result, freezing profiles are consistent from one run to the next. It is important to fully load CoolCell containers prior to freezing.  The CoolCell LX container foam retains very little heat which ensures that the container will impose negligible change in the freezer environment and protect nearby frozen samples. The low heat content also ensures that CoolCell LX containers will rapidly return to room temperature for another freezing cycle. A CoolCell FTS30 container utilizes a novel metal alloy and controlled micro-convection technology to evenly draw in -80°C freezer air through a bottom base vent, uniformly disperse the cold air around each vial in the central chamber and then release the thermal load from the vials through a top vent choke. The inner vial module holds 30 cryogenic vials and can be removed in one step. Each vial achieves a uniform and reproducible -1°C/minute freezing profile. The vial-to-vial and cycle-to-cycle thermal profiles are highly reproducible when the same vial load and freezer temperature is applied. Due to the low thermal mass of the uniform-density cross-linked polyethylene foam container, freezing can be conducted without a rise in local freezer temperature, thereby protecting nearby samples. The low thermal mass also ensures that CoolCell FTS30 containers will rapidly return to room temperature for another freezing cycle.

PERFORMANCE DATA

Click here for CoolCell performance data

APPLICATIONS

Cell Cryopreservation >
Cell Invasion and Migration >
Cell Therapy >
Biobanking >

PROTOCOLS

Cell and Stem Cell Cryopreservation >
PBMC Isolation >

 


  • CoolRack®, CoolSink® and ThermalTray™

    Thermo-conductive modules and platforms

     

    The thermo-conductive properties of the CoolRack, CoolSink and ThermalTray product lines enable safe, efficient and highly reproducible handling and processing of temperature-sensitive samples. When placed in direct contact with a temperature source such as ice, dry ice, liquid nitrogen, water baths, incubators, ovens and others, the modules will rapidly adapt to the source temperature. CoolRack tube modules and the patented CoolSink® plate modules and ThermalTray™ platforms fit into many facets of the workflows for cell and tissue culture and cryopreservation, cell therapy, biobanking sample preparation and transport, histology, immunohistochemistry, virus and bacterial research, molecular biology, biofuels research and others.



    PROBLEM: wet samples

    • Non-uniform ice contact results in variable sample temperature
    • Disorganized, wet samples and labels
    • Shifting, sinking tubes - contamination risk
    • Non-reproducible method

    SOLUTION: CoolRack

    • All samples <4˚C and  uniform in temperature (+/- 0.1˚C)
    • Samples are organized, secure and dry
    • All tubes are upright and indexed
    • Reproducible method

    With a CoolRack, CoolSink or ThermalTray interface between your valuable samples and the temperature source, you can minimize risk of contamination, ensure uniform cooling, freezing or warming to your samples, and keep your workflow organized.

     

    HOW IT WORKS

    CoolRack, CoolSink and ThermalTray are precision-engineered modules manufactured from a novel thermo-conductive alloy material that evenly distributes the temperature across all wells providing very uniform and consistent temperature to all samples (+/-1˚C). Thermo-conductivity is the transfer of heat energy from a higher temperature region to a lower temperature region.

    Cooling

    Warming


    CoolRack on Ice (left): Heat from the relatively warmer CoolRack sample module is transferred to cooling source (wet or dry ice, cartridge, or liquid nitrogen) until equilibrium is reached.
    CoolRack in Water Bath (right): Heat is transferred from water bath or other heat source toward relatively cooler CoolRack sample module until equilibrium is reached.

     

    PERFORMANCE DATA

    Click here for CoolRack, CoolSink and ThermalTray performance data

    APPLICATIONS

    Snap Freezing on Dry Ice or Liquid Nitrogen >

    PCR Preparation >
    Proteomics Sample Handling >
    Virus Freezing >
    Incubating and Thawing in a Water Bath >
    Cell Therapy >
    Biobanking >
    Ice-free Sample Cooling or Freezing >

    PROTOCOLS

    Virus Cryopreservation >
    Bacteria Cryopreservation >
    Tissue Snap Freezing >
    Cell and stem Cell Cryopreservation >
    PBMC Isolation >

     


    CoolBox™
    Portable ice-free cooling and freezing systems

     

    The elimination of ice from sample preparation and handing minimizes the risk of sample contamination, non-uniform cooling, wet labels, illegible markings, disorganization and sample mis-identification.  The proprietary and patent-pending XT Cooling Core technology gives CoolBox systems the cooling or freezing power needed to deliver all day bench top cooling or freezing without the use of ice in a flexible format that accommodates a variety of sample vessels.  

    CoolBox systems are compact and light-weight making them ideal for transporting samples from lab to lab, liquid nitrogren tank to biobank or for other short-term mobile requirements.  CoolBox system materials are compatible with use in a laminar flow hood or clean room.

    HOW IT WORKS

    Each CoolBox system consists of a base, XT cooling/freezing core or cartridge, and a lid. CoolBox XT workstations also include a magnetized collar and a 1 to 8˚C temperature indicator for visual assurance of temperature during use. Each CoolBox system is designed to receive thermo-conductive CoolRack and CoolSink tube and plate sample modules that sit directly on the core/cartridge and keep samples at desired temperature for hours. The core/cartridge is pre-frozen in a -20˚C freezer prior to placing in the CoolBox system.

    PERFORMANCE DATA

    Click here for CoolBox performance data

    APPLICATIONS

    PCR Preparation >
    Proteomics Sample Handling >
    Cell Therapy >
    Biobanking >
    Ice-free Sample Cooling or Freezing >

    PROTOCOLS
    Virus Cryopreservation >
    Bacteria Cryopreservation >
    Tissue Snap Freezing >
    Cell and stem Cell Cryopreservation >
    PBMC Isolation >