Standardized Cryopreservation of Human Primary Cells

Thomas V. Ramos, Aby J. Mathew, Maria L. Thompson, Rolf O. Ehrhardt

HemaCare Corporation, Van Nuys, California,  BioLife Solutions, Bothell, Washington
BioCision, Larkspur, California

Curr. Protoc. Cell Biol. 64:A.3I.1‐A.3I.8.


Download Abstract



Cryopreservation is the use of low temperatures to preserve structurally intact living cells. The cells that survive the thermodynamic journey from the 37°C incubator to the −196°C liquid nitrogen storage tank are free from the influences of time. Thus, cryopreservation is a critical component of cell culture and cell manufacturing protocols. Successful cryopreservation of human cells requires that the cells be derived from patient samples that are collected in a standardized manner, and carefully handled from blood draw through cell isolation. Furthermore, proper equipment must be in place to ensure consistency, reproducibility, and sterility. In addition, the correct choice and amount of cryoprotectant agent must be added at the correct temperature, and a controlled rate of freezing (most commonly 1°C/min) must be applied prior to a standardized method of cryogenic storage. This appendix describes how human primary cells can be frozen for long‐term storage and thawed for growth in a tissue culture vessel.


Featured in protocol:

CoolCell® alcohol-free cell freezing container

Learn more >


Other publications:

Cryopreservation and Thawing of Cells

Wayne M. Yokoyama, Maria L. Thompson, Rolf O. Ehrhardt
University of California School of Medicine, San Francisco, California

Curr Protoc Immunol. 2012 Nov;99 Appendix 3:3G



Standardized Cryopreservation of Pluripotent
Stem Cells

Rick I. Cohen, Maria L. Thompson,Brian Schryver, Rolf O. Ehrhardt
Rutgers University, Piscataway, New Jersey
BioCision LLC, Larkspur, California

Curr Protoc Stem Cell Bio. 2014 Feb; 28 Unit 1C.14











Contact BioCision:

About Us
Contact Us
Privacy Policy

© Copyright 2020, BioCision.
All rights reserved

BioCision is a life science research and development company that develops products and solutions for process standardization throughout the healthcare industry through the application of advanced thermal regulation principles and technologies. The intuitive design and interconnectivity of BioCision products enables researchers, clinicians and manufacturers to protect the integrity of temperature-sensitive therapeutics, biological samples, and biomaterials. By comprehensively addressing temperature stability, BioCision strives to improve the success of therapeutic discovery and development and enable effective care delivery.

Help 1-800-367-4887