Standardized Cryopreservation of Pluripotent Stem Cells

Cohen, R.I.,  Thompson, M.L.,  Schryver, B., and Ehrhardt R.O. (2015) 
Standardized Cryopreservation of Pluripotent Stem Cells
Curr. Protoc. Stem Cell Biol. 35:1C.14.1-1C.14.14. 

The successful exploitation of human cells for research, translational, therapeutic, and commercial purposes requires that effective and simple cryopreservation methods be applied for storage in local and master cell banks. Of all cell types utilized in modern research, human embryonic stem cells and their more recent relatives, induced pluripotent stem cells, are two of the most sensitive to cryopreservation. It is frequently observed that the lack of quality control and proper processing techniques yield poor recovery of pluripotent stem cells. The procedures in this unit have been optimized for handling some of the most recalcitrant stem cell lines, and provide a method for controlled-rate freezing, using minimal equipment that affords levels of cell viability comparable to expensive controlled-rate freezers, and standardized cell thawing. The protocol provides a clinically relevant, inexpensive, reliable, and user-friendly method that successfully prepares cells for long-term cold storage and ensures maximum levels of cell viability post thaw.



Other publications:

Cryopreservation and Thawing of Cells

Wayne M. Yokoyama, Maria L. Thompson, Rolf O. Ehrhardt
University of California School of Medicine, San Francisco, California

Curr Protoc Immunol. 2012 Nov;99 Appendix 3:3G



Standardized Cryopreservation of Human Primary Cells

HemaCare Corporation, Van Nuys, California, BioLife Solutions, Bothell, Washington, BioCision, Larkspur, California

Curr. Protoc. Cell Biol. 64:A.3I.1‐A.3I.8.